Biomass is one of the renewable sources of bioenergy, capable of contributing significantly to the future global energy supply . The anaerobic digestion process involving biological conversions in which a consortium of interdependent organisms responsible for the degradation of complex organic matter . It is important to evaluate appropriate techniques and / or technologies for effective energy recovery from waste. The effect of inoculums on the substrate ratio (I / S) in biogas production was investigated by changing the amount of substrate added to the inoculums. Process for the “production” of inoculate, the experiment was selected case, 14 days, 50°C, and constituted BATCH system, chicken waste, cattle waste, corn silage and corn (corn – without milled). Biogas production was the final step of the experiment. The “Batch Constant Temperature System” system used ration: 25gr. inoculums: 0,5gr. substrate. In the environmental experiment, the time period was 69 days (i.e. about 9 weeks). From this, it was noted that, despite the prolonged time, the maximum reached value was 68.33% CH4, while in laboratory conditions was ~70% CH4. The maximum value of biomethane produced reached for 56 days, while in laboratory conditions it reaches for 25-28 days.
Keywords: Biomass, inoculums, biomethane, substrate, biogas.