1Department of Plant Science and Technology, Faculty of Agriculture, Agricultural University of Tirana, Albania
The aim of this study was to analyse the chemical composition and the variability of essential oils obtained from seven native accessions of Origanum vulgare L. subs.vulgare taken from the Albanian Gene Bank and cultivated in the experimental field of the Agricultural University of Tirana. Essential oils were obtained by steam distillation and analysed using gas chromatography equipped with a flame ionization detector (GC-FID) and examined for their volatile constituents by gas chromatography-mass spectroscopy (GC-MS). The results showed that the yield of essential oils (v/w dry weight) varied depending on accessions and ranged from 0.08 to 0.33%. Forty-nine volatile constituents were identified in total. The main constituents were: Germacrene D (18.05-37.23%), E-Caryophyllene (12.80-21.50%), α-Cadinol (2.39-5.62%), Carvacrol (1.81-5.24%), Elemol (1.63-4.88%), BornylAcetate (0.82-6.09%), Thymol (0.41-8.06%), α-Humulene (2.20-3.35%), Sabinene (0.7-7.41%), E,E-α-Farnesene(1.31-4.54%), Caryophyllene Oxide (1.67-3.04%) and Z-β-Ocimene (0.13-6.89%).
Keywords: Origanum vulgare L. subs. vulgare, gas chromatography, clusters, essential oils.