Assessment of Saponins Stability in Liquid Extracts of Hedera helix and Primula veris by Using High Performance Thin Layer Chromatography Method

1Department of Pharmacy, Faculty of Medical Sciences, ALDENT University, Tirana, Albania
2Central Laboratory of Albanian Armed Forces, Tirana, Albania
*Corresponding author: Rezarta Shkreli;


In recent years, the interest in the herbal medicine use has increased worldwide. Herbal therapy has several
advantages over synthetic medicine, and the fact of being inexpensive with minimal side effects has accelerated
the demand for plant products. The aim of the study was to determine the contents of Hederacoside C in Hedera
helix leaves and Primula acid 1 in Primula veris roots as well as the stability of extracts under different storage
conditions. Four different extraction methods were performed: simple maceration, condenser-reflux maceration,
ultrasound maceration and maceration with magnetic mixer. The extracts were stored for two weeks under
different conditions of temperature, humidity and packaging containers. Quantitative assessments of
Hederacoside C and Primula acid 1 were performed immediately after extraction as well as after storage period,
by using CAMAG Linomat 4. The highest amount of extracted saponins resulted from extracts obtained from
the condenser-reflux maceration method: 2.97% for Hederacoside C and 5.82% for Primula acid 1. The losses
of Hederacoside C for 2 weeks of storage in different conditions were for the following: for maceration with
magnetic mixer method – in refrigerated condition, 8.1%; maceration method – in room condition, 10.2%;
condenser-reflux method – in coloured glass package, 7.7% and for maceration method – under high temperature
and humidity, 27.4%. The losses of Primula acid 1 for two weeks of storage in different conditions have resulted
in the following: maceration with condenser-reflux method in refrigerated condition, 4.7%; maceration method
– in room condition, 12.3%, condenser-reflux method – in coloured glass package, 7.7%, and maceration method
stored in high temperature and humidity condition, 21.6%. The stability of the active principles were affected
by storage conditions, so it is recommended to store them in a cool and dark place. This conclusion is significant
and may provide guidance on the phytochemicals used in oral form-dosage formulations.

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