REZARTA SHKRELI1*, KLITON VIDE2
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1 Department of Pharmacy, Faculty of Medical Sciences, ALDENT University, Tirana, Albania 2 Central Laboratory of Albanian Armed Forces, Tirana, Albania * Corresponding author: Rezarta Shkreli; rezarta.shkreli@ual.edu.al Abstract In recent years, the interest in the herbal medicine use has increased worldwide. Herbal therapy has several advantages over synthetic medicine, and the fact of being inexpensive with minimal side effects has accelerated the demand for plant products. The aim of the study was to determine the contents of Hederacoside C in Hedera helix leaves and Primula acid 1 in Primula veris roots as well as the stability of extracts under different storage conditions. Four different extraction methods were performed: simple maceration, condenser-reflux maceration, ultrasound maceration and maceration with magnetic mixer. The extracts were stored for two weeks under different conditions of temperature, humidity and packaging containers. Quantitative assessments of Hederacoside C and Primula acid 1 were performed immediately after extraction as well as after storage period, by using CAMAG Linomat 4. The highest amount of extracted saponins resulted from extracts obtained from the condenser-reflux maceration method: 2.97% for Hederacoside C and 5.82% for Primula acid 1. The losses of Hederacoside C for 2 weeks of storage in different conditions were for the following: for maceration with magnetic mixer method – in refrigerated condition, 8.1%; maceration method – in room condition, 10.2%; condenser-reflux method – in coloured glass package, 7.7% and for maceration method – under high temperature and humidity, 27.4%. The losses of Primula acid 1 for two weeks of storage in different conditions have resulted in the following: maceration with condenser-reflux method in refrigerated condition, 4.7%; maceration method – in room condition, 12.3%, condenser-reflux method – in coloured glass package, 7.7%, and maceration method stored in high temperature and humidity condition, 21.6%. The stability of the active principles were affected by storage conditions, so it is recommended to store them in a cool and dark place. This conc Keywords: hedera helix extract, primula veris extract, Hederacoside C, Primula acid 1, stability. |