ELEKTRA PAPAKOSTA1*, LUMTURI XHELILAJ2, ADHURIM LAZAJ3, ATHINA LLAMBRO4
1 Institute of Applied Agricultural Science, Nuertingen-Geislingen University, Neckarsteige 6-10, 72622 Nuertingen, Germany
2 Faculty of Agriculture, Economics and Management, Nuertingen-Geislingen University, Neckarsteige 6-10, 72622 Nuertingen, Germany
1.2.3.4 Centre of Agricultural Tecnology Transfer (ATTC) Vlore, Shamogjin, Albania
Download PDF Full-text
Abstract
GF-677 hybrid peach x almond hybrid (Prunus persica x Prunus amygdalus) is suitable in calcareous, nonirrigated, dry and hilly soils, susceptible to Agrobacterium, having good productivity. It is propagated using grafts and in vitro culture. The micropropagation of the peach rootstock aims to produce clean and certified phytosanitary fruit seedlings in terms of phytosanitary and genetic safety.
The aim of this study is to optimize the micropropagation protocols of GF-677 by evaluating the influence of chemical and physiological factors on the proliferation and rooting of the rootstock. Plant materials (apical buds) were sampled from the greenhouse with clean and controlled “mother” plants. The explants were disinfected with two sterilizing agents: NaOCl 0.5 % for 20 minutes, where the plants showed a high survival rate of 75%; and HgCl2 0.03 % where explants showed a lower survival rate of 44%. The effects of phytohormones in the proliferation phase were analyzed comparing different concentrations of BAP according to the scheme: 4 variants x 4 replications (0.5 mgl -1; 1 mg l -1; 1.5 mgl -1; 2 mgl – 1) with 15 plants each. The best results were obtained in the concentration of BAP 1mgl – 1 after three subcultures at the highest values of the average number of shoots 2.6 and shoot length 2.84 cm. In the proliferation phase, different nutrient media protocols were studied: DKW; MS; MS/2. The best results were recorded in the MS nutrient media (Murashige & Skoog, 1962) with the average number of shoots 5.78; average length of shoots 5.6cm; average number of leaves 8.9. The highest rooting index was achieved in MS nutrient media with average number of roots 5 and average root length 2.37 cm. After in vivo acclimatization of the plants, biometric parameters were measured to evaluate the grafting standard (d = 7mm) followed by grafting of the GF-677 with almond scion (Tuono) carried out. Grafting percentage resulted 70 % After budding, in the vegetative phase, were evaluated the data on the average length of shoot growth in April 40.5cm; May 71.6; June 103.2 cm and July 140.8 cm
Keywords: Growth regulator, sterilizing agents, media MS, tuono, cytokinin, auxin
|
