Comparision of Digestibility Estimation fFor Omd Through two In Vitro Methods for some Feeds, Used in Ruminant Animals

NERTILA DALIPAJ1,2,*, MARGARIDA R. G. MAIA2, ANA RITA J. CABRITA2, HUGO M. OLIVEIRA4, LUMTURI PAPA3, ANTÓNIO J. M. FONSECA2, ERINDA LIKA1

1 Department of Preclinical Subjects, Agricultural University of Tirana, KoderKamez 1001, Albania;
2 REQUIMTE, LAQV, ICBAS, Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal
3 Department of Animal Sciences, Agricultural University of Tirana, KoderKamez 1001, Albania,
4 INL, International Iberian Nanotechnology Laboratory, Avenida Mestre José Veiga s/n, 4715-330 Braga, Portugal;

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Abstract

Two in vitro procedures were used to assess the digestibility of some feeds used in ruminant feeding in Albania. In total nine samples from common used feeds in ruminant animals feeding, from which six cereal grains, two cereal straws and one ryegrass straw were analyzed for their chemical content of ash, crude protein (CP), crude fat (CF), neutral detergent fiber (NDF), acid detergent fiber(ADF), acid detergent lignin (ADL), acid detergent insoluble nitrogen (ADIN) and neutral detergent insoluble nitrogen (NDIN). All the samples were estimated for organic matter digestibility (OMD) through both Tilley&Terry and Pepsin-cellulase methods. Average protein content of cereal grains varied from 10.1 % for oat to 10.8% for barley. The ADF and ADL content varied respectively 3.90% -.17.6% and 0.80% -3.70%. The straws have similar chemical content for main Weende parameters and as expected average protein content was lower and varied from 2.60% for wheat to 3.60% for ryegrass while the ADF and ADL content varied respectively 53.8% (oat) – 56.3 (wheat) and 7.70% (ryegrass) – 8.70% (oat). The OMD determined with Tilley and Terry and pepsin cellulase methods resulted to be similar in straw samples while for cereals, the oat grain presented the lowest value obtained from both methods, 69.7% and 65.0%, respectively for TT and PC method.Tilley and Terry procedure gave higher values of OMD for all feed samples in comparison with enzymatic method, 68.4% vs 58.6%respectively for TT and PC method.The study revealed that digestibility results obtained by these two methods were highly correlated (r=0.99). According to R2 -value (0.98) the OMD determined by Tilley and Terry method could be predicted from enzymatic test as most convenient since it does not need animals.

Keywords: Tilley and Terry; Pepsine-celulase; organic matter digestibility; feeds