Effect of Anticoagulants on Some Biochemical Indicators of Cow Blood

JETMIRA ABESHI1*, ELENICA DIMCO1, ORNELA BALLA2, ERSEL DOKO3

1 Medicine, Agriculture University of Tirana, Albania

2 Department of Informatic, University “Aleksander Xhuvani” of Elbasan, Albania

3 Veterinary inspector, Regional Agency of Veterinary Service, Elbasan

* jabeshi@ubt.edu.al

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Abstract

This study presents the effect that some anticoagulants have on some biochemical indicators of the blood of cows. Blood samples for the purposes of this study are taken in the area of Elbasan, in the administrative unit of Paper. Approximately 20 ml of blood was taken from each of the cows, which was stored as follows: (i) 5ml stored in test tube without any anticoagulant substances, (ii) 5ml stored in test tube containing lithium heparin, (iii) 5ml stored in test tube containing K2EDTA, and (iii) 5 ml stored in test tube containing Na Citrate. The values obtained from measurements taken with serum have served as reference values in order to compare the figures obtained from samples mixed with other anticoagulants. The metabolites measured are bilirubin total, proteins total, creatinine, glucose, ALT, AST, GGT, potassium, chlorine and calcium. The evaluation of metabolic indicators is achieved using the semiautomatic Biochemical Analyzer EMP-168, through commercial kits “Human”.
The results have shown that the values of metabolites, enzymes, and macro-microelements measured in the plasma containing heparin are comparable to the values obtained from measurements with serum. Meanwhile, values of plasma metabolite stored with EDTA have shown a significant difference in elements as urea, protein total, and creatinine (p<0.01). Furthermore, a higher concentration of potassium was observed in samples stored with EDTA (p<0.001), while calcium demonstrated a significant decrease for samples stored with EDTA, and Na Citrates (p<0.001). Values of plasma metabolites stored with citrate have stated a significant reduction (p>0.001) for all the assessed metabolites, compared such with their values measured in serum.
In conclusion, based on the results obtained by this study and also other authors’ findings, it is important to state that, for routine biochemical analysis of cow blood, in addition to those in serum, the most reliable references are the samples when blood is stored with anticoagulant elements as heparin.

Keywords: Biochemistry, EDTA, Lithium heparin, Citrate Na, Cows