In silico analysis of osteocalcin gene in Cyprinidae

XHILIOLA BIXHEKU¹, ANILA HODA^2*, LORENA HYSI²,VILSON BOZGO²¹Quality Assurance Agency for Higher Education,

²Agricultural University of Tirana,

*Corresponding author e-mail: ahoda@ubt.edu.al;

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Abstract

Cyprinidae family includes a large number of fish species, where the most popular are zebrafish (Danio rerio), grass carp (Ctenopharyngodon idella) and common carp (Cyprinus carpio).  In this study, osteocalcin in Cyprinus carpio was investigated, regarding physicochemical characteristics, structural properties using several available bioinformatic tools. Sequences were retrieved from Genbank. Sequence alignment of the gene and protein sequence were done with ClustalW. The 3D structure of protein was predicted by SWISS-Model softare andwas checked by Z-Score using Qmean server, ERRAT, and Rampage Ramachandran plot analysis. The information provided here is a theoretical overview that will help to get an idea about the predicted protein structure.

Keywords: osteocalcin, bioinformatic tools, sequence alignment