BRUNILDA ÇUKO, VALBONA SOTA, EFIGJENI KONGJIKA
Biotechnology Department, Faculty of Natural Sciences, Tirana University, Tirana, Albania *
Section of Natural and Technical Sciences, Academy of Sciences, Tirana, Albania
Corresponding author e-mail: cuko.brunilda@gmail.com
Abstract
Rooting induction appears very difficult, especially regarding to trees species but it’s necessary to stabilize a protocol for in vitro rhizogenesis before the acclimatization stage. These study was carry out to investigate the influence of different doses of auxinic phytohormone NAA (α-naphthaleneacetic acid) and MS nutrients on in vitro rhizogenesis of apple (Malus domestica L.) which is a fruit extremely rich in important antioxidants, flavanoids, and dietary fiber. The induction and improvement of in vitro rhizogenesis of microshoots of two different apple cultivars (cv. Golden Delicious and cv. Starking ) was tested on three nutrient rooting media with different concentrations of NAA and macro- and micronutrients, presented in the universal medium MS: (I) – ½ MS macronutrients, MS micronutrients, MS vitamins supplemented with 0.1 mg l-1 NAA; (II) – ½ MS macronutrients, ½ MS micronutrients, MS vitamins with 0.1 mg l-1 NAA and (III) – MS macronutrients, ½ MS micronutrients, MS vitamins with 2 mg l-1 NAA). The first signs of roots formation were detected 10-12 days after inoculation of strongest microshoots of each cultivar of apple. There were detected significant differences in rooting percentage according to the rooting media and the highest value of rooting for both cultivars resulted in the first rooting medium (90.2%). There was not observed significant difference in rooting percentage between two studied cultivars. As the result the first medium with half concentration of MS macronutrients and 0.1 mg l-1 NAA is recommended.
Keywords: rhizogenesis, MS medium, NAA (1-Naphthaleneacetic acid), rooting percentage.
