Allelic frequencies of two microsatellite loci in four populations of brown trout (Salmo trutta)

EDIT VARDHAMI¹, ANILA HODA^1*, ADIOLA BIBA¹, MANUELA GUALTIERI², MASSIMO MECATTI², AGIM REXHEPI³

¹Department of Animal Production, Agricultural University of Tirana Albania.

²Department of Animal Production, Agricultural University of Florence Italia.

³University of Prishtina “Hasan Prishtina”, Faculty of Agriculture and Veterinary Kosovo.

*Corresponding author e-mail: ahoda@ubt.edu.al

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Abstract.

Two microsatellite loci, Str60Inra and Ssa197, were PCR amplified on 30 individuals for each populations of brown trout (Salmo trutta). A total of 120 individuals were selected from rivers of the Florence province (Italy), Valbona and Cen (Albania), Lepenci (Kosovo). There were identified 32 different alleles for Str60Inra and 41 for the locus Ssa197. Mean number of alleles ranged from 9 (Cen) to 20.5 (Florence). The mean observed and expected heterosygosities values were 0.329 and 0.755, respectively. Both microsatellite loci were polymorphic. The highest value of heterozygosity was observed in Lepenci. Significant deviations from Hardy Weinberg were found in both loci.

Keywords: microsatellite, polymorphism, observed and expected heterosygozity.