Evaluation of the correlation between pH and MPV platelet concentrates prepared in Tirana Blood Transfusion Center.

MERITA XHETANI^1,2, ARBEN METKA², IRENA SEFERI²

¹Faculty of Natural Sciences, Department of Biology, Blv. Zog I, Tirana, Albania

² National Blood Transfusion Center, Str. Lord Bajron, Tirana, Albania

* corresponding author e-mail:  merita.xhetani@fshn.edu.al

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Abstract

The quality of platelet concentrates is an important option in transfusion therapy. pH and platelet indices have been found to be valuable parameters for monitoring the in vitro quality of platelet concentrates. Platelet activation which leads to loss of its functionality has been demonstrated by changes in those two parameters. The aim of the study was to evaluate the correlation between pH and mean platelet volume (MPV) in platelet concentrates in order to examine the quality of platelet concentrate. 150 units of platelet concentrates were produced by platelet reach plasma (PRP), and stored for 5 days. Then MPV and pH were analyzed by automated hematological cell counter and Ph meter respectively. Regression analysis showed that there was a significant influence of pH changes on the changes in MPV (P<0.05). On the other hand, increase in pH lead to decrease in MPV. Storing platelet concentrates up to 5 days may stimulate platelet activity, enhancing its size and resulted in its destruction, so the remaining platelet are those with significantly lower MPV. Also platelet activation was those with an increase in pH. As a result measurements of MPV and pH have a great potential as quality markers of platelet concentrates.

Keywords: MPV, platelet concentrate, transfusion.