Adiola Biba, Anila Hoda*, Edit Vardhami
Department of Animal Production, Agricultural University of Tirana, Albania
*Corresponding author e-mail: ahoda@ubt.edu.al
Abstract
Common carp (Cyprinus carpio L) is an important fish species of Ohrid lake. The aim of the present study was to evaluate the genetic diversity of common carp using microsatellite markers. A total of 30 individuals were genotyped for 4 microsatellite loci (MFW1, MFW6, MFW7, MFW18). All the microsatellite loci were polymorphic. A total of 84 alleles were distinguished. The allelic number varied from 19 to 23 with mean allelic number of 21. The effective number of alleles varied from 9.92 to 11.84 with a mean of 11.66. The observed heterozygosity ranged from 0.32 to 0.73 with mean value of 0.57. The loci were highly informative. The PIC values ranged from 0.89 to 0.91 with mean of 0.90. The values of Shannon information index (I) ranges from 2.63 to 2.78. The population was not in Hardy-Weinberg equilibrium (HWE) for all of four loci. The mean expected heterosygosity value (0.91) was higher the mean observed heterosygosity (0.57). The mean fixation index (F) over the loci was 0.37. A bottleneck analysis is carried out, which shows no recent bottleneck. Factorial Correspondence Analysis (FCA) shows a close relationship between individuals.
Key words: common carp, microsatellite, heterosygosity, genetic diversity, polymorphic.
