Molecular identification by means of Seminested PCR of Dermanyssus gallinae circulating in Albania

TANA SHTYLLA KIKA1, MICHELE MARINO2, GIULIO COCCIOLO3, NICOLA PUGLIESE2 SONILA ÇOÇOLI1, JONIDA BOCI3, ANTONIO CAMARDA2

1Faculty of Veterinary Medicine, Agricultural University of Tirana, Albania

2Faculty of Veterinary Medicine, “Aldo Moro”University of Baru, Italy

3Institute of Food Safety and Food, Tirana, Albania

*Corresponding author  e-mail: shtylla@ubt.edu.al        

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Abstract:

In the present work a total of 5 sample sets of Poultry Red mites, collected in 5 different poultry (layers and broilers) farms of Albania, through the Cardboard method, were investigated. The mites previously macroscopically identified as Dermanyssus gallinae using morphological keys were investigated by molecular tools. These samples were analysed for the following identification of the ITS and COI genes by means of Seminested PCR. The SM PCR guaranties a major specificity and susceptibility. The phylogenetic identification of Dermanyssus gallinae was based on the detection of the ITS (internal transcribed spacers) gene and the specific genomic sequence COI (a portion of the cytochrome c oxidase gene of the mitochondrial DNA) gene. This study revealed no genetic diversities of D. gallinae distributed in Albania as all of the Sm PCRs assays resulted positive, where the 5 sample sets resulted positive (100%) either on the presence of ITS gene, so in the COI gene, which confirms them as Dermanyssus gallinae mites. This work represents a preliminary step for the implementation of larger epidemiologic investigation aimed to assess the extent of the circulation of D. gallinae in Albania, and to retrieve phylogenetic data about the mite populations.

Keywords: Dermanyssus gallinae, Albania, poultry farms, ITS gene, COI gene.

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