FAIK SERTEL SECER1*, ALEXANDER ATANASOFF2, DIMITRINKA ZAPRYANOVA3, GALIN NIKOLOV4, FERHAT CAGILTAY5
1Ankara University, Faculty of Agriculture / 06110 Ankara, Turkey
2Institute of Fish Resources / 9000 Varna, Bulgaria
3Trakia University, Faculty of Veterinary Medicine / 6014 Stara Zagora, Bulgaria
4Trakia University, Faculty of Agriculture / 6014 Stara Zagora, Bulgaria
5Istanbul University, Faculty of Fishery science / 34140 Istanbul, Turkey
*Corresponding author e-mail: email@example.com
In Bulgaria the production of high-quality local species is insignificant and is represented mainly by carp, grass carp and trout. Recently, diversification of the produced species has included specie such as African catfish (Clarias gariepinus). The present study was conducted to establish reference normal range of some hematological and biochemical parameters in wels sharptooth catfish (Clarias gariepinus) being reared in RAS, Bulgaria. To reduce stress during the blood sampling fish were anesthetized with mobile electro stunning device and blood was drawn with a syringe containing heparin as anticoagulant, by the caudal vein puncture method. The collected blood (approximately 2.0 ml) has been centrifuged at 3000 rpm for 5 min (Ohaus FC5515, Ohaus Corp., Germany) at room temperature and plasma was immediately separated and stored at -20°C until analysis. The different parameters were determined using standardized clinical methods and haematological techniques. The haematological parameters include the erythrocyte sedimentation rate (17.9±0.94), haemoglobin (25.96±4.58) and haematocrit (27.47±3.10). The mean values for plasma biochemical parameters – total protein (TP), albumin (Alb), globulin (Glb), urea (Ur), creatinine (Cr), aspartate transaminase (AST), alanine transaminase (ALT), glucose (Gl), calcium (Ca) and phosphorus (P) were 15.93±2.70 g/l; 8.28±2.02 g/l; 7.90±1.83 g/l; 0.67±0.18 µmol/l; 73.61±21.33 µmol/l; 73.78±28.06 UI; 14.71±3.67 UI; 1.76±0.36 µmol/l; 0.94±0.22 µmol/l respectivity. The haematological and biochemical reference intervals established may allow clinically important decisions in the assessment of health and well-being of this economically important African catfish (Clarias gariepinus) in Bulgarian aquaculture.
Keywords: Biochemical indices, catfish, hematological indices, reference intervals.