Determination of imidacloprid residues on tomatoes by High-Performance Liquid Chromatography.

VJOLLCA VLADI1*, FATOS HARIZAJ2, VALDETE VORPSI2, MAGDALENA CARA3

1Food Safety and Veterinary Institute. Tirana, Albania.

2Faculty of Agriculture and Environment, AUT, Department of Plant Production, Tirana, Albania

3Faculty of Agriculture and Environment, AUT, Department of Plant Protection, Tirana, Albania

*Corresponding author e-mail: vjollcavladi@hotmail.com

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Abstract

A simple method for the determination of imidacloprid residues in tomatoes, grown in greenhouses, has been developed. Two procedures for extraction (acetone/ethyl acetate; acetonitrile/methanol) of the analyte from the sample matrix are suggested. Glass wool and Florisil column chromatography were used for purification of sample solution. The technique used for detection was Liquid Chromatography equipped with UV detector. LCMS was used as a confirmatory method. The recoveries ranged from 92.4 – 98.8 % for acetone/ethyl acetate extraction and from 97.5- 99.1% for acetonitrile/methanol extraction. Tomatoes treated with imidacloprid using commercial insecticide formulation – Confidor were analyzed using both procedures. There are differences between the test results obtained by the two procedures at 5% significance level. The acetonitrile/methanol extraction is recommended for use at determination of imidacloprid in tomatoes.

Key words: HPLC analysis, Imidacloprid, Tomato, Pesticide Residues.

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