ELVIRA BAZINA1* BARRY MURPHY2 LIRI DINGA3
1 Natural Resources Sustainability and Export Markets Development Consultant/Tirana, Albania
2NGS Sequencing Service Manager, Source Bioscience/1 Orchard Place, Nottingham Business Park, NG8 6PX, Nottingham, UK
3University of Tirana, Faculty of Natural Sciences/Tirana, Albania
* Corresponding author e-mail: firstname.lastname@example.org
Albania continues to be a significant supplier of wild Medicinal and Aromatic Plants to the world markets of which Sage remains the major export item accounting for about 70% of the total sage imports to the US in 2013. Sage plants were randomly picked from different cultivation sites in Albania (North/Koplik; Southeast/Skrapar and South/Libohove) in order to screen genetic diversity amongst them employing Randomly Amplified Polymorphic DNA markers using twenty decameric oligonucleotide primers. A total of 2132 DNA bands were generated of which notably clear and scorable were 1555 (from 150 to 1999bp). Primers produced between 63 and 156 bands per Sage plant with an average of 107 bands per primer. Cultivated Sage plant generated between 112 to166 DNA bands with an average of 143 bands per plant. DNA banding patterns, obtained from the Shimadzu Multina PCR-RAPD analysis, were quite polymorphic and were used to carry out hierarchical cluster analysis using the average linkage between groups method of SPSS version 22. The dendrogram showed splitting of the North cultivated Sage from the Southern (southeast and south) group due to (dis)similarity in climate and soil structure/texture. Southeast cultivated Sage plants exhibited some genetic diversity within the group (intrinsic factors driven). This study indicates that RAPDs were fast and easy to use and proved to be efficient discriminatory tools detecting a high level of polymorphism within the same species (intraspecific level) which is explained with ecological variation and the genetic make-up of each individual.
Keywords: Sage; cultivation; Albania; PCR-RAPDs; electropherogram, dendrogram.